Cytotoxicity and Histopathology of Novel Bioceramic Root ‎Repair Materials Versus MTA Angelus: An in-vitro study

Aly, Yousra; Elsherif, Sammar

doi:10.21608/asdj.2025.331582.1606

Cytotoxicity and Histopathology of Novel Bioceramic Root ‎Repair Materials Versus MTA Angelus: An in-vitro study

Document Type : Original articles

Authors

Yousra Aly ¹
Sammar Elsherif ²

¹ Restorative and Dental materials research Department, National Research Centre, Dokki, Egypt

² Researcher of Endodontics Restorative and Dental materials research department, National Research Centre

10.21608/asdj.2025.331582.1606

Abstract

Aim: The aim of this in vitro study was to evaluate the cytotoxic and histopathologic effects induced by NeoPutty, experimental Nano-Mineral Trioxide Aggregate (Nano-MTA) compared to MTA-Angelus on human gingival fibroblasts (HGFs).
Materials and Methods: Material extracts were prepared at (24, 48 and 72 hours) at different serial dilution (undiluted, 50%, 25%, 12.5%). Human Gingival Fibroblast post-treated with pure extract of each of the three tested materials were examined under light microscope to detect the presence of apoptosis.
Results: At all evaluation periods, all tested materials significantly decreased the viability percentage of the fibroblast compared to control group at (concentrations 100%,50%); while at concentrations 25%,12.5%), all materials were non-cytotoxic with no significant difference with the control group. At 24 & 48 hours (concentrations 100% and 50%), MTA Angelus presented the highest cytotoxicity significantly to the other groups. At 24h (concentration 100%), Nano-MTA showed the least cytotoxicity significantly to MTA Angelus & NeoPutty, while at (concentration 50%) there was no significant difference in cytotoxicity between Nano-MTA & NeoPutty. At 48h, there was no significant difference in cytotoxicity between NeoPutty and Nano-MTA. At 72h (concentrations 100% & 50%), MTA Angelus presented the lowest cytotoxicity significantly to NeoPutty with no significant difference with Nano-MTA. Apoptosis was shown with the three tested materials.
Conclusion: Within the limitation of the present study, NeoPutty and experimental Nano-MTA had favorable cell viability that could be comparable to the gold standard MTA Angelus.

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