Effect of Addition of Omega-3 Fatty Acids to Nano-‎Hydroxyapatite on its Physical Properties: An In-Vitro Study

Gareer, Amira; Elsewify, Tarek Medhat; Nagy, Mohamed Mokhtar; El-Ashry, Salma Hassan; Hashem, Ahmed Abd Elrahman

doi:10.21608/asdj.2024.300214.1343

Effect of Addition of Omega-3 Fatty Acids to Nano-‎Hydroxyapatite on its Physical Properties: An In-Vitro Study

Document Type : Original articles

Authors

¹ Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.

² 1- Department of Restorative Dental Sciences, College of Dentistry, Gulf Medical University, Ajman, UAE. 2- Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.

³ 1- Department of Endodontics, Faculty of Dentistry, Al Galala University, Suez, Egypt. 2- Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.

⁴ Department of Endodontics, Faculty of Dentistry, Ain Shams University, Cairo, Egypt

10.21608/asdj.2024.300214.1343

Abstract

Aim: This study evaluated the physical properties of nano-hydroxyapatite (nHA) after the addition of Omega-3 (ꞷ3) powder in terms of radiopacity, solubility & pH.
Materials and methods: Radiopacity of group I (nHA), group II (2nHA:1ꞷ3), & group III (1nHA:1ꞷ3) were digitally evaluated against aluminium step wedge, human dentin & bovine cortical bone. Solubility of Calcium (Ca²⁺) was evaluated after 1,3 & 5 days in Tris-HCL solution of 7.4 pH & Citric Acid solution after 5 days using atomic absorption spectroscopy. pH was evaluated along with the solubility test in Tris-HCL at 0h, 1, 3 & 5 days.
Results: The highest radiopacity level was recorded in nHA group. While groups containing ꞷ3 showed the least mean grey value. Solubility of Ca²⁺ ions increased with the increase in immersion time & with the decrease in the pH of the solution. In both solutions, group III showed the greatest mean of Ca²⁺ concentration released followed by group II & group I during all observation periods. In Tris-HCL, initial rise in pH up to 7.94 was observed in group I at 0h followed by a gradual drop reaching a pH value of 6.72 at day 5. While in groups II & III, pH showed an initial decrease at 0h, a gradual increase followed from day 1 to 5.
Conclusion: Adding (ꞷ3) to (nHA) lowered its radiopacity and increased its solubility in Tris-HCL & acidic medium. (nHA) calcium release was not affected by low pH, offering a clinical advantage in case of bacterial infection.

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