Effect of caffeine versus hydrogen peroxide on human skin fibroblast cell line cytotoxicity, cell cycle phases, and apoptosis.

Document Type : Original articles

Authors

1 Lecturer of Oral Pathology Faculty of Oral & Dental Medicine, Badr University in Cairo (BUC), Egypt.

2 Professor of Oral Biology, Faculty of Dentistry, Mansoura University, Egypt.

3 Chair of Oral Biology & Pathology Department Faculty of Oral & Dental Medicine, Badr University in Cairo (BUC), Egypt.

Abstract

Aim: The study reported the effect of caffeine and H2O2, concerning cytotoxicity followed by cell cycle analysis and programmed cell death against the human skin fibroblast cell line (HSF).
Materials and Methods: HSF treated by 1.6 mM concentration sub-lethal dose of H2O2 and caffeine with various concentrations. A flow cytometry test was accomplished to estimate the effect of caffeine and H2O2 on the HSF cell line.
Results: This study postulated that caffeine had a lower cytotoxicity effect on the HSF cell line with IC50>5 µM in contrast to H2O2 showed cytotoxicity above 1.6 µM. As compared to the control cells, caffeine-treated cells showed a significant decline in the early and late apoptotic cell content by -60% and -48%, respectively. However, H2O2-treated cells exhibited significant elevations in the early apoptotic content as compared to the control cells.
Conclusions: The study showed that H2O2 had an effect of cytotoxicity on HSF and arrested more cells in the S phase in comparison to caffeine. Caffeine is safer on the HSF cell line.

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