Down-regulation of MicroRNA-137 Improves Proliferation of Oral Squamous Cell Carcinoma by Up-regulation of NF-Y

Document Type : Original articles

Authors

1 Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University Assiut ,Egypt

2 Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University

3 Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Minia, Egypt

4 Oral and Maxillofacial Pathology, Faculty of Dentistry, minia University,minia,Egypt

Abstract

ABSTRACT:
Aim: The purpose of our study was to investigate the impact of miR-137 suppression on the growth of the OECM-1 human tongue SCC cell line. In addition, by targeting the NF-Y gene, we also studied the molecular mechanism of miR-137.
Methods: The (OECM-1) & negative control (SCC-15) cells were transfected with microRNA137 inhibitor, MTT assay was utilized to ascertain the cytotoxic impact. SYBER green-based quantitative polymerase chain reaction was used to determine the expression level of both the miR-137 and NF-Y genes in treated and untreated cells. The collected data were subjected to One-way analysis of variance (ANOVA) and Tukey's multiple comparisons test.
RESULTS: According to our research, OECM-1 cells transfected with an inhibitor of miR-137 significantly increased their ability to proliferate when compared to an untreated samples & negative control. Also, we reported that miR-137 is down regulated in OSCC and suppression of miR-137 significantly associated with 1.4 folds increase in NF-Y gene, compared to untreated OECM-1 cells.
CONCLUSION: MiR-137 is down regulated in OSCC cell lines compared to normal control & its inhibition has a significant increase in cell proliferation which reflects the tumor suppressor role of miR-137 in OSCC. In addition, its inhibition is associated with marked increase in the expression of NF-Y which suggested NF-Y a new target for miR-137.

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