Expression of Matrix Metalloproteinase-9, P53 and B-cl2 Genes in Human Osteosarcoma Cell Line Treated with Ginger Extract

Document Type : Original articles

Authors

1 Demonstrator of Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University, Egypt.

2 Associate Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt.

3 Former Head of Research and Development Sector of The Egyptian Holding Company of Vaccines, Sera and Drug (Egy-Vac, VACSERA-Egypt).

4 Lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Egypt.

Abstract

Aim: The current study was designed to explore the potential effect of ginger extract on ‎the osteosarcoma cell line (MG-63).‎
Material and Methods: Human osteosarcoma cell line (MG-63) was acquired from ‎Nawah-Scientific. The tested drug was ginger extract prepared by maceration in semi-‎liquid form.   The potential impact of ginger extract upon MG-63 cells was evaluated ‎using cytotoxicity assay, microscopic evaluation, Enzyme-Linked Immunosorbent ‎Assay for evaluation of Matrix Metalloproteinase-9 (MMP-9), and Real-Time ‎Polymerase Chain Reaction for detection of the expression levels of the pro-apoptotic ‎gene (P53) and antiapoptotic gene B-cell lymphoma 2 (B-cl2) in ginger extract-treated ‎cells. Then the data collected was statistically assessed.‎
Results: The outcomes demonstrated that ginger extract's cytotoxic impact ‎upon MG-63 cells was dose-dependent, as viability increased as long as the ‎concentration decreased. Under the microscope, MG-63 cells treated with ‎ginger extract displayed apoptotic signs such as chromatin condensation, cell ‎shrinkage, and plasma membrane blebbing. Data about MMP-9 showed that, ‎ginger extract dramatically and dose-dependently reduced MMP-9 levels. ‎Regarding P53 and B-cl2, data revealed that ginger extract significantly ‎increased P53 levels and decreased B-cl2 in a dose-dependent manner.‎
Conclusion: Ginger extract substantially diminishes the viability of osteosarcoma ‎cells because of its strong cytotoxic activity against MG-63 cells. ‎
 

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Ain Shams Dental Journal
Volume 35, Issue 3
September 2024
Pages 232-243

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