Antibacterial Efficacy of Chitosan ‎Nanoparticles-‎Incorporated with Different Endodontic ‎Irrigation Solutions: ‎An In-Vitro Study

Ali, Sara Flaah; Aljadwaa, Fanar Turki; Hamdoon, Suhad Mwafaq

doi:10.21608/asdj.2025.335356.1634

Antibacterial Efficacy of Chitosan ‎Nanoparticles-‎Incorporated with Different Endodontic ‎Irrigation Solutions: ‎An In-Vitro Study

Document Type : Original articles

Authors

¹ Department of Conservative Dentistry, College of Dentistry, University of Mosul, Mosul, Iraq

² Department of Dental Basic Sciences, College of Dentistry, University of Mosul, Mosul, Iraq

10.21608/asdj.2025.335356.1634

Abstract

Aim: ‎ The Purpose of this investigation was to evaluate antimicrobial effectiveness of ‎two concentrations of nanochitosan (NC 0.2%, 0.5%) combined with sodium ‎hypochlorite (NaOCl 5.25%) and chlorhexidine (CHX 2%).
Materials and methods: Seventy two ‎‎,without caries, single rooted mandibular first premolars with developed apices ‎extracted for orthodontic purposes used. The roots of all teeth will be sectioned 14mm ‎from the tip of the root. The entire root surface was covered by two thin layers of nail ‎polish, and the apical ends of the roots were sealed with flowable composite. All ‎samples were prepared using the Protaper Ni-Ti rotary system.Ten microliters (10 µl) of ‎Enterococcus faecalis suspension were injected inside root canals and incubated for 48 ‎hours.Roots were divided at random into nine groups (n = 8).Group I: NC 0.2%, Group ‎II: NC 0.5%, Group III: NaOCl 5.25%, Group IV: CHX 2%, Group V: NC 0.2% + NaOCl ‎‎5.25%, Group VI: NC 0.5% + NaOCl 5.25%, Group VII: NC 0.2% + CHX 2%, Group ‎VIII: NC 0.5% + CHX 2%, Group IX: Distilled Water(D.W.). After disinfecting the ‎canal, intracanal bacterial samples were collected and counted in order to establish the ‎number of colony-forming units (CFUs).
Result: According to the study, a statistically ‎significant difference was observed in mean of CFUs of the experimental groups and ‎control group. Highest antibacterial activity was when NC was mixed with NaOCl. ‎
Conclusion: This research demonstrates that there is synergistic antimicrobial activity ‎when NC irrigation solution (0.2%, 0.5%) is mixed with NaOCl 5.25% and CHX 2%.‎

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