Document Type : Original articles
Authors
1
Villa 103, Street 17, 5th Settlement, New Cairo, Cairo, Egypt
2
Associate Professor, Department of Oral Biology, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
3
Assistant lecturer, Oral and maxillofacial surgery, Faculty of Oral and Dental Medicine, Future University in Egypt, Cairo, 11835, Egypt;
4
oral pathology department, future university in Egypt, Cairo, Egypt
Abstract
Aim: Matcha, a vibrant green tea powder has gained popularity as a trendy beverage due to its potent antioxidant properties. E-cigarettes, despite increasing consumption, especially among young adults, have been linked to health deterioration. This study investigated the potential of matcha tea extract to mitigate the harmful effects of e-cigarette liquid on human fibroblast cells.
Materials and methods: Human fibroblast cells were divided into four groups: control, matcha tea extract only, e-cigarette liquid only, and a combination of both. Cytotoxic concentrations were determined by MTT assay, and cell morphology, death, cycle, protein expression (Bax/Bcl2), and ultrastructure were analyzed.
Results: Flowcytometry analysis revealed a decrease in necrotic and apoptotic cell populations in cells treated with both matcha tea extract and e-cigarette liquid compared to those exposed to e-cigarette liquid only. Ultrastructural examination revealed that necrotic morphology was most prominent in the e-cigarette liquid only group. Cell cycle analysis indicated a slightly higher accumulation of cells in the G2/M phase in the e-cigarette liquid only group compared to the combination group, while the combination group had a higher percentage of cells in the S phase.
Conclusion: While matcha tea extract may not be a complete solution, it offers promising insights into the potential of natural compounds to mitigate the harmful effects of e-cigarettes. Further research is essential to fully understand its mechanisms of action and explore its clinical relevance.
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