The Anticancer Effect of Piperine on Tongue Squamous Cell Carcinoma Cell Line

Document Type : Original articles

Authors

1 Assistant lecturer of Oral and Maxillofacial pathology, Sphinx University

2 Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University

Abstract

Aim: The purpose of this study was to investigate the impact of piperine (PP) on tongue squamous cell carcinoma cell line.
Materials and Methods: In this research, cultured tongue carcinoma cells were separated into two groups: a control group, and PP treated group. The cytotoxicity assay was utilized to measure cell viability. Flow cytometry was employed to examine and assess the cell cycle and apoptosis phases.
Results: Piperine induced cytotoxicity on tongue carcinoma cells, reducing the mean percentage of cell viability in a concentration-dependent manner, with an IC50 of 21.2 μM. Moreover, PP treatment induced a rise in the percentage of cells in the G1 to S phases of the cell cycle and a reduction in the percentage of cancer cells in the G2/M phase. Exposure of tongue carcinoma cells to PP led to a decrease in the number of viable cells, along with a rise in the combined proportion of apoptotic and necrotic cells to 31.14%.
Conclusion: Piperine reduced the viability, caused cell cycle changes, and induced cell death of tongue squamous cell carcinoma cell line suggesting that PP has a potential as a therapeutic agent.

Keywords

Main Subjects

Files

Share

How to cite

Statistics