Histopathological Assessment of the Safety and Efficiency of Hyaluronic Acid Filler Augmentation in Rats’ Labial Dermal Matrix (Histological, Histochemical & Immunohistochemical Study)

Document Type : Original articles

Authors

1 Oral biology Department, Faculty of dentistry, Ain Shams University, Cairo, Egypt.

2 Oral biology department, Mansoura University & Egyptian Russian Uni. Cairo, Egypt

10.21608/asdj.2025.341418.1694

Abstract

Aim: to assess the safety and efficacy of HA in restoring the labial volume and shape while maintaining its structural integrity.
Materials and methods: 30 rats were equally divided into two main groups; Group I (Control): Included fifteen adult rats injected subcutaneously with saline. Group ІІ: experimental groups, included fifteen rats whose lower lip mucosae were injected with 0.02 ml HA filler. Each of the control and experimental groups were equally subdivided into three subgroups, Subgroup A: sacrificed 7 days after injection. Subgroup B: sacrificed 14 days after injection. Subgroup C: sacrificed 28 days after injection. Lip specimens were processed for routine H&E examination, histochemical examination using Alcian blue & Picro Sirious Red stain, as well as Immunohistochemical assessment using anti-cox2 .
Results: experimental groups displayed more vacuolated epithelial cells, more inflammatory cell infiltration, more cox-2 positive cells and more collagen hyalinization within the connective tissue .
Conclusion: HA filler induced a moderate foreign body reaction whose intensity increased with time.

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